Review




Structured Review

Santa Cruz Biotechnology pc10
Pc10, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 6710 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcna+pc10+antibody/PCNA+Antibody/pmc12813500-95-19-20
Average 96 stars, based on 6710 article reviews
pc10 - by Bioz Stars, 2026-09
96/100 stars

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Related Articles

Infection:

Article Title: The Impact of Non-Lethal Single-Dose Radiation on Tumor Invasion and Cytoskeletal Properties
Article Snippet: Anti-GAPDH (37 kDa) antibody (Cell Signaling, Cambridge, UK, 14C10, 1:1000) was used as housekeeping protein and PCNA (Santa Cruz, Dallas, TX, USA, 1:1000, PC10, 36 kDa) for the assessment of proliferation.

Article Title: IFI16 senses and protects stalled replication forks
Article Snippet: Western blotting : GAPDH Antibody (SCBT, G-9, 1:2000), RPA2 (Abcam 9H8, ab2175, 1:1000), Tubulin (Abcam, ab4074), SMARCAL1 (SCBT, sc-376377), γH2AX (Cell Signaling Technology, 9718), PCNA (SCBT, PC10, sc-56, 1:1000), H3 (Cell Signaling Technology, 9715, 1:1000), IFI16 (SCBT, 1G7, sc-8023, 1:1000); cGAS (Cell Signaling Technology, E5V3W, 1:1000), STING (Cell Signaling Technology, D2P2F, 1:1000), β-actin (Sigma A2228, 1:3000).

Article Title: Suppressing mitochondrial inner membrane protein (IMMT) inhibits the proliferation of breast cancer cells through mitochondrial remodeling and metabolic regulation
Article Snippet: PCNA (PC10; dilution,1:800) and CCND1 (A-12; dilution, 1:800) were purchased from Santa Cruz Biotechnology. β-Actin (M1210-2; dilution, 1:5000) were purchased from HUABIO.

Article Title: Tat expression led to increased histone 3 tri-methylation at lysine 27 and contributed to HIV latency in astrocytes through regulation of MeCP2 and Ezh2 expression
Article Snippet: PCNA (PC10) antibody and MeCP2 (H300) antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Article Title: A single non-synonymous NCOA5 variation in type 2 diabetic patients with hepatocellular carcinoma impairs the function of NCOA5 in cell cycle regulation
Article Snippet: PCNA (PC10, 1:100, sc-56, Santa Cruz Biotechnology) and γH2AX (pSer139, 1:100) antibody were used.

Article Title: A Peptide Mimicking a Region in Proliferating Cell Nuclear Antigen Specific to Key Protein Interactions Is Cytotoxic to Breast Cancer
Article Snippet: Membranes were blocked for 30 minutes in buffer containing 10 mM Tris-HCl (pH 7.5), 100 mM NaCl, 2.5 mM EDTA (pH 8.0), 0.1% Tween 20, and 5% nonfat dry milk, then exposed to either PCNA PC10 (1:2000 dilution) (Santa Cruz Biotechnology, Dallas, TX) or the epitope-mapping anti-rabbit caPCNA antibodies (1:500 dilution) for 90 minutes.

Article Title: E2F-dependent transcription determines replication capacity and S phase length
Article Snippet: PCNA PC10 sc-56, MCM2 sc9839, MCM7 sc56324 from Santa Cruz and MCM3 A300-192A from Bethyl Laboratories, anti-mouse Alexa Fluor 488 from Life Technologies.

Western Blot:

Article Title: The Impact of Non-Lethal Single-Dose Radiation on Tumor Invasion and Cytoskeletal Properties
Article Snippet: Anti-GAPDH (37 kDa) antibody (Cell Signaling, Cambridge, UK, 14C10, 1:1000) was used as housekeeping protein and PCNA (Santa Cruz, Dallas, TX, USA, 1:1000, PC10, 36 kDa) for the assessment of proliferation.

Article Title: IFI16 senses and protects stalled replication forks
Article Snippet: Western blotting : GAPDH Antibody (SCBT, G-9, 1:2000), RPA2 (Abcam 9H8, ab2175, 1:1000), Tubulin (Abcam, ab4074), SMARCAL1 (SCBT, sc-376377), γH2AX (Cell Signaling Technology, 9718), PCNA (SCBT, PC10, sc-56, 1:1000), H3 (Cell Signaling Technology, 9715, 1:1000), IFI16 (SCBT, 1G7, sc-8023, 1:1000); cGAS (Cell Signaling Technology, E5V3W, 1:1000), STING (Cell Signaling Technology, D2P2F, 1:1000), β-actin (Sigma A2228, 1:3000).

Article Title: Suppressing mitochondrial inner membrane protein (IMMT) inhibits the proliferation of breast cancer cells through mitochondrial remodeling and metabolic regulation
Article Snippet: PCNA (PC10; dilution,1:800) and CCND1 (A-12; dilution, 1:800) were purchased from Santa Cruz Biotechnology. β-Actin (M1210-2; dilution, 1:5000) were purchased from HUABIO.

Article Title: Tat expression led to increased histone 3 tri-methylation at lysine 27 and contributed to HIV latency in astrocytes through regulation of MeCP2 and Ezh2 expression
Article Snippet: PCNA (PC10) antibody and MeCP2 (H300) antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Article Title: A single non-synonymous NCOA5 variation in type 2 diabetic patients with hepatocellular carcinoma impairs the function of NCOA5 in cell cycle regulation
Article Snippet: PCNA (PC10, 1:100, sc-56, Santa Cruz Biotechnology) and γH2AX (pSer139, 1:100) antibody were used.

Article Title: A Peptide Mimicking a Region in Proliferating Cell Nuclear Antigen Specific to Key Protein Interactions Is Cytotoxic to Breast Cancer
Article Snippet: Membranes were blocked for 30 minutes in buffer containing 10 mM Tris-HCl (pH 7.5), 100 mM NaCl, 2.5 mM EDTA (pH 8.0), 0.1% Tween 20, and 5% nonfat dry milk, then exposed to either PCNA PC10 (1:2000 dilution) (Santa Cruz Biotechnology, Dallas, TX) or the epitope-mapping anti-rabbit caPCNA antibodies (1:500 dilution) for 90 minutes.

Article Title: E2F-dependent transcription determines replication capacity and S phase length
Article Snippet: PCNA PC10 sc-56, MCM2 sc9839, MCM7 sc56324 from Santa Cruz and MCM3 A300-192A from Bethyl Laboratories, anti-mouse Alexa Fluor 488 from Life Technologies.

Quantitation Assay:

Article Title: The Impact of Non-Lethal Single-Dose Radiation on Tumor Invasion and Cytoskeletal Properties
Article Snippet: Anti-GAPDH (37 kDa) antibody (Cell Signaling, Cambridge, UK, 14C10, 1:1000) was used as housekeeping protein and PCNA (Santa Cruz, Dallas, TX, USA, 1:1000, PC10, 36 kDa) for the assessment of proliferation.

Article Title: IFI16 senses and protects stalled replication forks
Article Snippet: Western blotting : GAPDH Antibody (SCBT, G-9, 1:2000), RPA2 (Abcam 9H8, ab2175, 1:1000), Tubulin (Abcam, ab4074), SMARCAL1 (SCBT, sc-376377), γH2AX (Cell Signaling Technology, 9718), PCNA (SCBT, PC10, sc-56, 1:1000), H3 (Cell Signaling Technology, 9715, 1:1000), IFI16 (SCBT, 1G7, sc-8023, 1:1000); cGAS (Cell Signaling Technology, E5V3W, 1:1000), STING (Cell Signaling Technology, D2P2F, 1:1000), β-actin (Sigma A2228, 1:3000).

Article Title: Suppressing mitochondrial inner membrane protein (IMMT) inhibits the proliferation of breast cancer cells through mitochondrial remodeling and metabolic regulation
Article Snippet: PCNA (PC10; dilution,1:800) and CCND1 (A-12; dilution, 1:800) were purchased from Santa Cruz Biotechnology. β-Actin (M1210-2; dilution, 1:5000) were purchased from HUABIO.

Article Title: Tat expression led to increased histone 3 tri-methylation at lysine 27 and contributed to HIV latency in astrocytes through regulation of MeCP2 and Ezh2 expression
Article Snippet: PCNA (PC10) antibody and MeCP2 (H300) antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Article Title: A single non-synonymous NCOA5 variation in type 2 diabetic patients with hepatocellular carcinoma impairs the function of NCOA5 in cell cycle regulation
Article Snippet: PCNA (PC10, 1:100, sc-56, Santa Cruz Biotechnology) and γH2AX (pSer139, 1:100) antibody were used.

Article Title: A Peptide Mimicking a Region in Proliferating Cell Nuclear Antigen Specific to Key Protein Interactions Is Cytotoxic to Breast Cancer
Article Snippet: Membranes were blocked for 30 minutes in buffer containing 10 mM Tris-HCl (pH 7.5), 100 mM NaCl, 2.5 mM EDTA (pH 8.0), 0.1% Tween 20, and 5% nonfat dry milk, then exposed to either PCNA PC10 (1:2000 dilution) (Santa Cruz Biotechnology, Dallas, TX) or the epitope-mapping anti-rabbit caPCNA antibodies (1:500 dilution) for 90 minutes.

Article Title: E2F-dependent transcription determines replication capacity and S phase length
Article Snippet: PCNA PC10 sc-56, MCM2 sc9839, MCM7 sc56324 from Santa Cruz and MCM3 A300-192A from Bethyl Laboratories, anti-mouse Alexa Fluor 488 from Life Technologies.

Quantitative RT-PCR:

Article Title: The Impact of Non-Lethal Single-Dose Radiation on Tumor Invasion and Cytoskeletal Properties
Article Snippet: Anti-GAPDH (37 kDa) antibody (Cell Signaling, Cambridge, UK, 14C10, 1:1000) was used as housekeeping protein and PCNA (Santa Cruz, Dallas, TX, USA, 1:1000, PC10, 36 kDa) for the assessment of proliferation.

Article Title: IFI16 senses and protects stalled replication forks
Article Snippet: Western blotting : GAPDH Antibody (SCBT, G-9, 1:2000), RPA2 (Abcam 9H8, ab2175, 1:1000), Tubulin (Abcam, ab4074), SMARCAL1 (SCBT, sc-376377), γH2AX (Cell Signaling Technology, 9718), PCNA (SCBT, PC10, sc-56, 1:1000), H3 (Cell Signaling Technology, 9715, 1:1000), IFI16 (SCBT, 1G7, sc-8023, 1:1000); cGAS (Cell Signaling Technology, E5V3W, 1:1000), STING (Cell Signaling Technology, D2P2F, 1:1000), β-actin (Sigma A2228, 1:3000).

Article Title: Suppressing mitochondrial inner membrane protein (IMMT) inhibits the proliferation of breast cancer cells through mitochondrial remodeling and metabolic regulation
Article Snippet: PCNA (PC10; dilution,1:800) and CCND1 (A-12; dilution, 1:800) were purchased from Santa Cruz Biotechnology. β-Actin (M1210-2; dilution, 1:5000) were purchased from HUABIO.

Article Title: Tat expression led to increased histone 3 tri-methylation at lysine 27 and contributed to HIV latency in astrocytes through regulation of MeCP2 and Ezh2 expression
Article Snippet: PCNA (PC10) antibody and MeCP2 (H300) antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Article Title: A single non-synonymous NCOA5 variation in type 2 diabetic patients with hepatocellular carcinoma impairs the function of NCOA5 in cell cycle regulation
Article Snippet: PCNA (PC10, 1:100, sc-56, Santa Cruz Biotechnology) and γH2AX (pSer139, 1:100) antibody were used.

Article Title: A Peptide Mimicking a Region in Proliferating Cell Nuclear Antigen Specific to Key Protein Interactions Is Cytotoxic to Breast Cancer
Article Snippet: Membranes were blocked for 30 minutes in buffer containing 10 mM Tris-HCl (pH 7.5), 100 mM NaCl, 2.5 mM EDTA (pH 8.0), 0.1% Tween 20, and 5% nonfat dry milk, then exposed to either PCNA PC10 (1:2000 dilution) (Santa Cruz Biotechnology, Dallas, TX) or the epitope-mapping anti-rabbit caPCNA antibodies (1:500 dilution) for 90 minutes.

Article Title: E2F-dependent transcription determines replication capacity and S phase length
Article Snippet: PCNA PC10 sc-56, MCM2 sc9839, MCM7 sc56324 from Santa Cruz and MCM3 A300-192A from Bethyl Laboratories, anti-mouse Alexa Fluor 488 from Life Technologies.

Expressing:

Article Title: The Impact of Non-Lethal Single-Dose Radiation on Tumor Invasion and Cytoskeletal Properties
Article Snippet: Anti-GAPDH (37 kDa) antibody (Cell Signaling, Cambridge, UK, 14C10, 1:1000) was used as housekeeping protein and PCNA (Santa Cruz, Dallas, TX, USA, 1:1000, PC10, 36 kDa) for the assessment of proliferation.

Article Title: IFI16 senses and protects stalled replication forks
Article Snippet: Western blotting : GAPDH Antibody (SCBT, G-9, 1:2000), RPA2 (Abcam 9H8, ab2175, 1:1000), Tubulin (Abcam, ab4074), SMARCAL1 (SCBT, sc-376377), γH2AX (Cell Signaling Technology, 9718), PCNA (SCBT, PC10, sc-56, 1:1000), H3 (Cell Signaling Technology, 9715, 1:1000), IFI16 (SCBT, 1G7, sc-8023, 1:1000); cGAS (Cell Signaling Technology, E5V3W, 1:1000), STING (Cell Signaling Technology, D2P2F, 1:1000), β-actin (Sigma A2228, 1:3000).

Article Title: Suppressing mitochondrial inner membrane protein (IMMT) inhibits the proliferation of breast cancer cells through mitochondrial remodeling and metabolic regulation
Article Snippet: PCNA (PC10; dilution,1:800) and CCND1 (A-12; dilution, 1:800) were purchased from Santa Cruz Biotechnology. β-Actin (M1210-2; dilution, 1:5000) were purchased from HUABIO.

Article Title: Tat expression led to increased histone 3 tri-methylation at lysine 27 and contributed to HIV latency in astrocytes through regulation of MeCP2 and Ezh2 expression
Article Snippet: PCNA (PC10) antibody and MeCP2 (H300) antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Article Title: A single non-synonymous NCOA5 variation in type 2 diabetic patients with hepatocellular carcinoma impairs the function of NCOA5 in cell cycle regulation
Article Snippet: PCNA (PC10, 1:100, sc-56, Santa Cruz Biotechnology) and γH2AX (pSer139, 1:100) antibody were used.

Article Title: A Peptide Mimicking a Region in Proliferating Cell Nuclear Antigen Specific to Key Protein Interactions Is Cytotoxic to Breast Cancer
Article Snippet: Membranes were blocked for 30 minutes in buffer containing 10 mM Tris-HCl (pH 7.5), 100 mM NaCl, 2.5 mM EDTA (pH 8.0), 0.1% Tween 20, and 5% nonfat dry milk, then exposed to either PCNA PC10 (1:2000 dilution) (Santa Cruz Biotechnology, Dallas, TX) or the epitope-mapping anti-rabbit caPCNA antibodies (1:500 dilution) for 90 minutes.

Article Title: E2F-dependent transcription determines replication capacity and S phase length
Article Snippet: PCNA PC10 sc-56, MCM2 sc9839, MCM7 sc56324 from Santa Cruz and MCM3 A300-192A from Bethyl Laboratories, anti-mouse Alexa Fluor 488 from Life Technologies.

Control:

Article Title: The Impact of Non-Lethal Single-Dose Radiation on Tumor Invasion and Cytoskeletal Properties
Article Snippet: Anti-GAPDH (37 kDa) antibody (Cell Signaling, Cambridge, UK, 14C10, 1:1000) was used as housekeeping protein and PCNA (Santa Cruz, Dallas, TX, USA, 1:1000, PC10, 36 kDa) for the assessment of proliferation.

Article Title: IFI16 senses and protects stalled replication forks
Article Snippet: Western blotting : GAPDH Antibody (SCBT, G-9, 1:2000), RPA2 (Abcam 9H8, ab2175, 1:1000), Tubulin (Abcam, ab4074), SMARCAL1 (SCBT, sc-376377), γH2AX (Cell Signaling Technology, 9718), PCNA (SCBT, PC10, sc-56, 1:1000), H3 (Cell Signaling Technology, 9715, 1:1000), IFI16 (SCBT, 1G7, sc-8023, 1:1000); cGAS (Cell Signaling Technology, E5V3W, 1:1000), STING (Cell Signaling Technology, D2P2F, 1:1000), β-actin (Sigma A2228, 1:3000).

Article Title: Suppressing mitochondrial inner membrane protein (IMMT) inhibits the proliferation of breast cancer cells through mitochondrial remodeling and metabolic regulation
Article Snippet: PCNA (PC10; dilution,1:800) and CCND1 (A-12; dilution, 1:800) were purchased from Santa Cruz Biotechnology. β-Actin (M1210-2; dilution, 1:5000) were purchased from HUABIO.

Article Title: Tat expression led to increased histone 3 tri-methylation at lysine 27 and contributed to HIV latency in astrocytes through regulation of MeCP2 and Ezh2 expression
Article Snippet: PCNA (PC10) antibody and MeCP2 (H300) antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Article Title: A single non-synonymous NCOA5 variation in type 2 diabetic patients with hepatocellular carcinoma impairs the function of NCOA5 in cell cycle regulation
Article Snippet: PCNA (PC10, 1:100, sc-56, Santa Cruz Biotechnology) and γH2AX (pSer139, 1:100) antibody were used.

Article Title: A Peptide Mimicking a Region in Proliferating Cell Nuclear Antigen Specific to Key Protein Interactions Is Cytotoxic to Breast Cancer
Article Snippet: Membranes were blocked for 30 minutes in buffer containing 10 mM Tris-HCl (pH 7.5), 100 mM NaCl, 2.5 mM EDTA (pH 8.0), 0.1% Tween 20, and 5% nonfat dry milk, then exposed to either PCNA PC10 (1:2000 dilution) (Santa Cruz Biotechnology, Dallas, TX) or the epitope-mapping anti-rabbit caPCNA antibodies (1:500 dilution) for 90 minutes.

Article Title: E2F-dependent transcription determines replication capacity and S phase length
Article Snippet: PCNA PC10 sc-56, MCM2 sc9839, MCM7 sc56324 from Santa Cruz and MCM3 A300-192A from Bethyl Laboratories, anti-mouse Alexa Fluor 488 from Life Technologies.

Transfection:

Article Title: The Impact of Non-Lethal Single-Dose Radiation on Tumor Invasion and Cytoskeletal Properties
Article Snippet: Anti-GAPDH (37 kDa) antibody (Cell Signaling, Cambridge, UK, 14C10, 1:1000) was used as housekeeping protein and PCNA (Santa Cruz, Dallas, TX, USA, 1:1000, PC10, 36 kDa) for the assessment of proliferation.

Article Title: IFI16 senses and protects stalled replication forks
Article Snippet: Western blotting : GAPDH Antibody (SCBT, G-9, 1:2000), RPA2 (Abcam 9H8, ab2175, 1:1000), Tubulin (Abcam, ab4074), SMARCAL1 (SCBT, sc-376377), γH2AX (Cell Signaling Technology, 9718), PCNA (SCBT, PC10, sc-56, 1:1000), H3 (Cell Signaling Technology, 9715, 1:1000), IFI16 (SCBT, 1G7, sc-8023, 1:1000); cGAS (Cell Signaling Technology, E5V3W, 1:1000), STING (Cell Signaling Technology, D2P2F, 1:1000), β-actin (Sigma A2228, 1:3000).

Article Title: Suppressing mitochondrial inner membrane protein (IMMT) inhibits the proliferation of breast cancer cells through mitochondrial remodeling and metabolic regulation
Article Snippet: PCNA (PC10; dilution,1:800) and CCND1 (A-12; dilution, 1:800) were purchased from Santa Cruz Biotechnology. β-Actin (M1210-2; dilution, 1:5000) were purchased from HUABIO.

Article Title: Tat expression led to increased histone 3 tri-methylation at lysine 27 and contributed to HIV latency in astrocytes through regulation of MeCP2 and Ezh2 expression
Article Snippet: PCNA (PC10) antibody and MeCP2 (H300) antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Article Title: A single non-synonymous NCOA5 variation in type 2 diabetic patients with hepatocellular carcinoma impairs the function of NCOA5 in cell cycle regulation
Article Snippet: PCNA (PC10, 1:100, sc-56, Santa Cruz Biotechnology) and γH2AX (pSer139, 1:100) antibody were used.

Article Title: A Peptide Mimicking a Region in Proliferating Cell Nuclear Antigen Specific to Key Protein Interactions Is Cytotoxic to Breast Cancer
Article Snippet: Membranes were blocked for 30 minutes in buffer containing 10 mM Tris-HCl (pH 7.5), 100 mM NaCl, 2.5 mM EDTA (pH 8.0), 0.1% Tween 20, and 5% nonfat dry milk, then exposed to either PCNA PC10 (1:2000 dilution) (Santa Cruz Biotechnology, Dallas, TX) or the epitope-mapping anti-rabbit caPCNA antibodies (1:500 dilution) for 90 minutes.

Article Title: E2F-dependent transcription determines replication capacity and S phase length
Article Snippet: PCNA PC10 sc-56, MCM2 sc9839, MCM7 sc56324 from Santa Cruz and MCM3 A300-192A from Bethyl Laboratories, anti-mouse Alexa Fluor 488 from Life Technologies.

Cell Culture:

Article Title: The Impact of Non-Lethal Single-Dose Radiation on Tumor Invasion and Cytoskeletal Properties
Article Snippet: Anti-GAPDH (37 kDa) antibody (Cell Signaling, Cambridge, UK, 14C10, 1:1000) was used as housekeeping protein and PCNA (Santa Cruz, Dallas, TX, USA, 1:1000, PC10, 36 kDa) for the assessment of proliferation.

Article Title: IFI16 senses and protects stalled replication forks
Article Snippet: Western blotting : GAPDH Antibody (SCBT, G-9, 1:2000), RPA2 (Abcam 9H8, ab2175, 1:1000), Tubulin (Abcam, ab4074), SMARCAL1 (SCBT, sc-376377), γH2AX (Cell Signaling Technology, 9718), PCNA (SCBT, PC10, sc-56, 1:1000), H3 (Cell Signaling Technology, 9715, 1:1000), IFI16 (SCBT, 1G7, sc-8023, 1:1000); cGAS (Cell Signaling Technology, E5V3W, 1:1000), STING (Cell Signaling Technology, D2P2F, 1:1000), β-actin (Sigma A2228, 1:3000).

Article Title: Suppressing mitochondrial inner membrane protein (IMMT) inhibits the proliferation of breast cancer cells through mitochondrial remodeling and metabolic regulation
Article Snippet: PCNA (PC10; dilution,1:800) and CCND1 (A-12; dilution, 1:800) were purchased from Santa Cruz Biotechnology. β-Actin (M1210-2; dilution, 1:5000) were purchased from HUABIO.

Article Title: Tat expression led to increased histone 3 tri-methylation at lysine 27 and contributed to HIV latency in astrocytes through regulation of MeCP2 and Ezh2 expression
Article Snippet: PCNA (PC10) antibody and MeCP2 (H300) antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Article Title: A single non-synonymous NCOA5 variation in type 2 diabetic patients with hepatocellular carcinoma impairs the function of NCOA5 in cell cycle regulation
Article Snippet: PCNA (PC10, 1:100, sc-56, Santa Cruz Biotechnology) and γH2AX (pSer139, 1:100) antibody were used.

Article Title: A Peptide Mimicking a Region in Proliferating Cell Nuclear Antigen Specific to Key Protein Interactions Is Cytotoxic to Breast Cancer
Article Snippet: Membranes were blocked for 30 minutes in buffer containing 10 mM Tris-HCl (pH 7.5), 100 mM NaCl, 2.5 mM EDTA (pH 8.0), 0.1% Tween 20, and 5% nonfat dry milk, then exposed to either PCNA PC10 (1:2000 dilution) (Santa Cruz Biotechnology, Dallas, TX) or the epitope-mapping anti-rabbit caPCNA antibodies (1:500 dilution) for 90 minutes.

Article Title: E2F-dependent transcription determines replication capacity and S phase length
Article Snippet: PCNA PC10 sc-56, MCM2 sc9839, MCM7 sc56324 from Santa Cruz and MCM3 A300-192A from Bethyl Laboratories, anti-mouse Alexa Fluor 488 from Life Technologies.

Irradiation:

Article Title: The Impact of Non-Lethal Single-Dose Radiation on Tumor Invasion and Cytoskeletal Properties
Article Snippet: Anti-GAPDH (37 kDa) antibody (Cell Signaling, Cambridge, UK, 14C10, 1:1000) was used as housekeeping protein and PCNA (Santa Cruz, Dallas, TX, USA, 1:1000, PC10, 36 kDa) for the assessment of proliferation.

Article Title: IFI16 senses and protects stalled replication forks
Article Snippet: Western blotting : GAPDH Antibody (SCBT, G-9, 1:2000), RPA2 (Abcam 9H8, ab2175, 1:1000), Tubulin (Abcam, ab4074), SMARCAL1 (SCBT, sc-376377), γH2AX (Cell Signaling Technology, 9718), PCNA (SCBT, PC10, sc-56, 1:1000), H3 (Cell Signaling Technology, 9715, 1:1000), IFI16 (SCBT, 1G7, sc-8023, 1:1000); cGAS (Cell Signaling Technology, E5V3W, 1:1000), STING (Cell Signaling Technology, D2P2F, 1:1000), β-actin (Sigma A2228, 1:3000).

Article Title: Suppressing mitochondrial inner membrane protein (IMMT) inhibits the proliferation of breast cancer cells through mitochondrial remodeling and metabolic regulation
Article Snippet: PCNA (PC10; dilution,1:800) and CCND1 (A-12; dilution, 1:800) were purchased from Santa Cruz Biotechnology. β-Actin (M1210-2; dilution, 1:5000) were purchased from HUABIO.

Article Title: Tat expression led to increased histone 3 tri-methylation at lysine 27 and contributed to HIV latency in astrocytes through regulation of MeCP2 and Ezh2 expression
Article Snippet: PCNA (PC10) antibody and MeCP2 (H300) antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Article Title: A single non-synonymous NCOA5 variation in type 2 diabetic patients with hepatocellular carcinoma impairs the function of NCOA5 in cell cycle regulation
Article Snippet: PCNA (PC10, 1:100, sc-56, Santa Cruz Biotechnology) and γH2AX (pSer139, 1:100) antibody were used.

Article Title: A Peptide Mimicking a Region in Proliferating Cell Nuclear Antigen Specific to Key Protein Interactions Is Cytotoxic to Breast Cancer
Article Snippet: Membranes were blocked for 30 minutes in buffer containing 10 mM Tris-HCl (pH 7.5), 100 mM NaCl, 2.5 mM EDTA (pH 8.0), 0.1% Tween 20, and 5% nonfat dry milk, then exposed to either PCNA PC10 (1:2000 dilution) (Santa Cruz Biotechnology, Dallas, TX) or the epitope-mapping anti-rabbit caPCNA antibodies (1:500 dilution) for 90 minutes.

Article Title: E2F-dependent transcription determines replication capacity and S phase length
Article Snippet: PCNA PC10 sc-56, MCM2 sc9839, MCM7 sc56324 from Santa Cruz and MCM3 A300-192A from Bethyl Laboratories, anti-mouse Alexa Fluor 488 from Life Technologies.

Staining:

Article Title: The Impact of Non-Lethal Single-Dose Radiation on Tumor Invasion and Cytoskeletal Properties
Article Snippet: Anti-GAPDH (37 kDa) antibody (Cell Signaling, Cambridge, UK, 14C10, 1:1000) was used as housekeeping protein and PCNA (Santa Cruz, Dallas, TX, USA, 1:1000, PC10, 36 kDa) for the assessment of proliferation.

Article Title: IFI16 senses and protects stalled replication forks
Article Snippet: Western blotting : GAPDH Antibody (SCBT, G-9, 1:2000), RPA2 (Abcam 9H8, ab2175, 1:1000), Tubulin (Abcam, ab4074), SMARCAL1 (SCBT, sc-376377), γH2AX (Cell Signaling Technology, 9718), PCNA (SCBT, PC10, sc-56, 1:1000), H3 (Cell Signaling Technology, 9715, 1:1000), IFI16 (SCBT, 1G7, sc-8023, 1:1000); cGAS (Cell Signaling Technology, E5V3W, 1:1000), STING (Cell Signaling Technology, D2P2F, 1:1000), β-actin (Sigma A2228, 1:3000).

Article Title: Suppressing mitochondrial inner membrane protein (IMMT) inhibits the proliferation of breast cancer cells through mitochondrial remodeling and metabolic regulation
Article Snippet: PCNA (PC10; dilution,1:800) and CCND1 (A-12; dilution, 1:800) were purchased from Santa Cruz Biotechnology. β-Actin (M1210-2; dilution, 1:5000) were purchased from HUABIO.

Article Title: Tat expression led to increased histone 3 tri-methylation at lysine 27 and contributed to HIV latency in astrocytes through regulation of MeCP2 and Ezh2 expression
Article Snippet: PCNA (PC10) antibody and MeCP2 (H300) antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Article Title: A single non-synonymous NCOA5 variation in type 2 diabetic patients with hepatocellular carcinoma impairs the function of NCOA5 in cell cycle regulation
Article Snippet: PCNA (PC10, 1:100, sc-56, Santa Cruz Biotechnology) and γH2AX (pSer139, 1:100) antibody were used.

Article Title: A Peptide Mimicking a Region in Proliferating Cell Nuclear Antigen Specific to Key Protein Interactions Is Cytotoxic to Breast Cancer
Article Snippet: Membranes were blocked for 30 minutes in buffer containing 10 mM Tris-HCl (pH 7.5), 100 mM NaCl, 2.5 mM EDTA (pH 8.0), 0.1% Tween 20, and 5% nonfat dry milk, then exposed to either PCNA PC10 (1:2000 dilution) (Santa Cruz Biotechnology, Dallas, TX) or the epitope-mapping anti-rabbit caPCNA antibodies (1:500 dilution) for 90 minutes.

Article Title: E2F-dependent transcription determines replication capacity and S phase length
Article Snippet: PCNA PC10 sc-56, MCM2 sc9839, MCM7 sc56324 from Santa Cruz and MCM3 A300-192A from Bethyl Laboratories, anti-mouse Alexa Fluor 488 from Life Technologies.



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( A ) Representative 2D maximum projections of 3D-SIM images of an early and mid S-phase nucleus after labelling with the standard pulsing scheme and post-fixation <t>PCNA</t> immunofluorescence staining, scale bar = 5 µm. ( B ) Bar plot showing the mean correlation coefficient (Pearson’s R value with no threshold) between indicated image channels, as determined by the FIJI plugin Coloc 2, n = 4. Error bars represent SD. ( C ) Examples of nuclear areas containing individual replication events, with arrows indicating inferred directionality, scale bar = 500 nm. For each area, an EdU only, Cy3-xx-dUTP only, PCNA only and merged channel image are shown below. ( D ) Bar plot showing the mean nuclear integrated intensities (a.u.) of pH2AX and pRPA for cells treated as described in 1E, F, respectively. Three replicates per condition, error bars represent SD. ( E ) Representative 2D maximum projections of 3D-SIM images of cells after three different treatments: EdU for 30-min only, EdU for 30-min and Cy3-xx-dUTP for 30 (4 + 26)-min or 2-h HU treatment prior to labelling with EdU for 30-min followed by Cy3-xx-dUTP for 30 (4 + 26)-min. All conditions underwent post-fixation immunofluorescence staining of pH2AX, scale bars: 10 µm. The nuclear perimeter is annotated with a white dashed line, and a selected area (white square) is shown in the merged image, scale bars: 2 µm. ( F ) Representative 2D maximum projections of 3D-SIM images of cells after three different treatments: EdU for 30-min only, EdU for 30-min and Cy3-xx-dUTP for 30 (4 + 26)-min or 2-h HU treatment prior to labelling with EdU for 30-min followed by Cy3-xx-dUTP for 30 (4 + 26)-min. All conditions underwent post-fixation immunofluorescence staining of pRPA, scale bars: 10 µm. The nuclear perimeter is annotated with a white dashed line, and a selected area (white square) is shown in the merged image, scale bars: 2 µm. .
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Image Search Results


( A ) Representative 2D maximum projections of 3D-SIM images of an early and mid S-phase nucleus after labelling with the standard pulsing scheme and post-fixation PCNA immunofluorescence staining, scale bar = 5 µm. ( B ) Bar plot showing the mean correlation coefficient (Pearson’s R value with no threshold) between indicated image channels, as determined by the FIJI plugin Coloc 2, n = 4. Error bars represent SD. ( C ) Examples of nuclear areas containing individual replication events, with arrows indicating inferred directionality, scale bar = 500 nm. For each area, an EdU only, Cy3-xx-dUTP only, PCNA only and merged channel image are shown below. ( D ) Bar plot showing the mean nuclear integrated intensities (a.u.) of pH2AX and pRPA for cells treated as described in 1E, F, respectively. Three replicates per condition, error bars represent SD. ( E ) Representative 2D maximum projections of 3D-SIM images of cells after three different treatments: EdU for 30-min only, EdU for 30-min and Cy3-xx-dUTP for 30 (4 + 26)-min or 2-h HU treatment prior to labelling with EdU for 30-min followed by Cy3-xx-dUTP for 30 (4 + 26)-min. All conditions underwent post-fixation immunofluorescence staining of pH2AX, scale bars: 10 µm. The nuclear perimeter is annotated with a white dashed line, and a selected area (white square) is shown in the merged image, scale bars: 2 µm. ( F ) Representative 2D maximum projections of 3D-SIM images of cells after three different treatments: EdU for 30-min only, EdU for 30-min and Cy3-xx-dUTP for 30 (4 + 26)-min or 2-h HU treatment prior to labelling with EdU for 30-min followed by Cy3-xx-dUTP for 30 (4 + 26)-min. All conditions underwent post-fixation immunofluorescence staining of pRPA, scale bars: 10 µm. The nuclear perimeter is annotated with a white dashed line, and a selected area (white square) is shown in the merged image, scale bars: 2 µm. .

Journal: The EMBO Journal

Article Title: Spatial mapping of DNA synthesis reveals dynamics and geometry of human replication nanostructures

doi: 10.1038/s44318-025-00574-2

Figure Lengend Snippet: ( A ) Representative 2D maximum projections of 3D-SIM images of an early and mid S-phase nucleus after labelling with the standard pulsing scheme and post-fixation PCNA immunofluorescence staining, scale bar = 5 µm. ( B ) Bar plot showing the mean correlation coefficient (Pearson’s R value with no threshold) between indicated image channels, as determined by the FIJI plugin Coloc 2, n = 4. Error bars represent SD. ( C ) Examples of nuclear areas containing individual replication events, with arrows indicating inferred directionality, scale bar = 500 nm. For each area, an EdU only, Cy3-xx-dUTP only, PCNA only and merged channel image are shown below. ( D ) Bar plot showing the mean nuclear integrated intensities (a.u.) of pH2AX and pRPA for cells treated as described in 1E, F, respectively. Three replicates per condition, error bars represent SD. ( E ) Representative 2D maximum projections of 3D-SIM images of cells after three different treatments: EdU for 30-min only, EdU for 30-min and Cy3-xx-dUTP for 30 (4 + 26)-min or 2-h HU treatment prior to labelling with EdU for 30-min followed by Cy3-xx-dUTP for 30 (4 + 26)-min. All conditions underwent post-fixation immunofluorescence staining of pH2AX, scale bars: 10 µm. The nuclear perimeter is annotated with a white dashed line, and a selected area (white square) is shown in the merged image, scale bars: 2 µm. ( F ) Representative 2D maximum projections of 3D-SIM images of cells after three different treatments: EdU for 30-min only, EdU for 30-min and Cy3-xx-dUTP for 30 (4 + 26)-min or 2-h HU treatment prior to labelling with EdU for 30-min followed by Cy3-xx-dUTP for 30 (4 + 26)-min. All conditions underwent post-fixation immunofluorescence staining of pRPA, scale bars: 10 µm. The nuclear perimeter is annotated with a white dashed line, and a selected area (white square) is shown in the merged image, scale bars: 2 µm. .

Article Snippet: If immunostaining applied, fixed samples were incubated overnight at 4 °C with a primary antibody: phospho-histone H2A.X [Ser139] (1:800, Cell Signalling Technology, #2577); phospho-RPA32 [S33] (1:2000, Bethyl Laboratories, #A300-246A); or PCNA (PC10) (1:400, Santa Cruz, sc-56) in blocking media.

Techniques: Immunofluorescence, Staining

( A ) Bar plot showing the mean nuclear integrated intensities (a.u.) of MCM2, PCNA and EdU for asynchronous cells treated with Doxycycline for 24 h and DMSO control. G1 and S population were selected based on DAPI vs EdU. Three replicates per condition, error bars represent S.E.M (for each condition, at least 10,000 cells were analysed for G1 portion and at least 2000 cells were analysed for the S portion).

Journal: The EMBO Journal

Article Title: Spatial mapping of DNA synthesis reveals dynamics and geometry of human replication nanostructures

doi: 10.1038/s44318-025-00574-2

Figure Lengend Snippet: ( A ) Bar plot showing the mean nuclear integrated intensities (a.u.) of MCM2, PCNA and EdU for asynchronous cells treated with Doxycycline for 24 h and DMSO control. G1 and S population were selected based on DAPI vs EdU. Three replicates per condition, error bars represent S.E.M (for each condition, at least 10,000 cells were analysed for G1 portion and at least 2000 cells were analysed for the S portion).

Article Snippet: If immunostaining applied, fixed samples were incubated overnight at 4 °C with a primary antibody: phospho-histone H2A.X [Ser139] (1:800, Cell Signalling Technology, #2577); phospho-RPA32 [S33] (1:2000, Bethyl Laboratories, #A300-246A); or PCNA (PC10) (1:400, Santa Cruz, sc-56) in blocking media.

Techniques: Control